Alexandra Hillebrand-Voiculescu
CS - Danubius
Publicatii
Publication | Authors | Date | |
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conference
Ice Microbiome: From Antarctic Glaciers To Alpine Ice Caves |
Cristina Purcarea;Corina Itcus; Constantin Marin; Soon Gyu Hong; Victoria I. Paun; Aurel Persoiu; Paris Lavin; Traian Brad; Alexandra Hillebrand-Voiculescu; Denisa Pascu; Cristian Coman; Iris Tusa; Manuela E. Sidoroff | Polar 2018 – Xxxv Scar Biennial Meetings – Open Science Conference, Davos, Elveția, 2018 | |
Rezumat |
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article
Fungi In Perennial Ice From Scarisoara Ice Cave (Romania) |
Brad Traian; Itcus Corina; Pascu Madalina-Denisa; Persoiu Aurel; Hillebrand-Voiculescu Alexandra; Iancu Lavinia; Purcarea Cristina | Scientific Reports, 2018 | |
RezumatScreening of 1,000-years old ice layers from the perennial ice block of Scarisoara Ice Cave (NW Romania) revealed the presence of fungal communities. Using culture-dependent methods and molecular techniques based on DGGE fingerprinting of 18S rRNA gene fragments and sequencing, we identified 50 cultured and 14 uncultured fungi in presently-forming, 400 and 900 years old ice layers, corresponding to 28 distinct operational taxonomic units (OTUs). The dominant ice-contained fungal OTUs were related to Ascomycota, Basidiomycota and Cryptomycota phyla. Representatives of Mucoromycota and Chytridiomycota were also isolated from recent and 400 years old ice samples. The cryophilic Mrakia stokesii was the most abundant fungal species found in the cave ice samples of all prospected ages, alongside other cryophilic fungi also identified in various glacial environments. Ice deposits formed during the Little Ice Age (dated between AD 1,250 and 1,850) appeared to have a higher fungal diversity than the ice layer formed during the Medieval Warm Period (prior to AD 1,250). A more complex fungal community adapted to low temperatures was obtained from all analyzed ice layers when cultivated at 4 degrees C as compared to 15 degrees C, suggesting the dominance of cold-adapted fungi in this glacial habitat. The fungal distribution in the analyzed cave ice layers revealed the presence of unique OTUs in different aged-formed ice deposits, as a first hint for putative further identification of fungal biomarkers for climate variations in this icy habitat. This is the first report on fungi from a rock-hosted cave ice block. |
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article
Fungi In Perennial Ice From Scǎrişoara Ice Cave (Romania) |
Brad T.; Itcus C.; Pascu M.-D.; Perşoiu A.; Hillebrand-Voiculescu A.; Iancu L.; Purcarea C. | Scientific Reports, 2018 | |
RezumatScreening of 1,000-years old ice layers from the perennial ice block of Scǎrişoara Ice Cave (NW Romania) revealed the presence of fungal communities. Using culture-dependent methods and molecular techniques based on DGGE fingerprinting of 18S rRNA gene fragments and sequencing, we identified 50 cultured and 14 uncultured fungi in presently-forming, 400 and 900 years old ice layers, corresponding to 28 distinct operational taxonomic units (OTUs). The dominant ice-contained fungal OTUs were related to Ascomycota, Basidiomycota and Cryptomycota phyla. Representatives of Mucoromycota and Chytridiomycota were also isolated from recent and 400 years old ice samples. The cryophilic Mrakia stokesii was the most abundant fungal species found in the cave ice samples of all prospected ages, alongside other cryophilic fungi also identified in various glacial environments. Ice deposits formed during the Little Ice Age (dated between AD 1,250 and 1,850) appeared to have a higher fungal diversity than the ice layer formed during the Medieval Warm Period (prior to AD 1,250). A more complex fungal community adapted to low temperatures was obtained from all analyzed ice layers when cultivated at 4 °C as compared to 15 °C, suggesting the dominance of cold-adapted fungi in this glacial habitat. The fungal distribution in the analyzed cave ice layers revealed the presence of unique OTUs in different aged-formed ice deposits, as a first hint for putative further identification of fungal biomarkers for climate variations in this icy habitat. This is the first report on fungi from a rock-hosted cave ice block. © 2018 The Author(s). |