The objectives of this project are to synthesise two molecularly imprinted polymers (MIPs) and to use them in the development of two analytical methods for the detection of nitrofuran class drugs/metabolites. The illegal use of nitrofuran class drugs (furazolidone, furaltadone, nitrofurantoin, nitrofurazone, etc.) as growth promoters in animal husbandry leads to the emergence of new strains of dangerous antibiotic-resistant bacteria and produces dangerous metabolites, which remain for a long time in meat. EU regulations require the control of relevant biological samples taken from live animals (urine, blood), meat from slaughterhouses or traders and prepared food. Current analytical techniques are time-consuming and costly due to the complexity of the sample matrix and the low concentration of analytes. The two MIPs synthesised in this project will be used in two different analytical applications: (1) the development of affinity-based sensors and (2) for sample processing by solid phase extraction (SPE) prior to chromatographic analysis. The sensors will be used for rapid screening of biological samples and constructed by immobilisation of the MIPs on a translator and detection will be performed with a suitable technique such as electrochemical impedance spectroscopy (EIS) or quartz crystal microbalance (QCM). The main advantages of sample processing with MIPs in chromatographic analysis are isolation and concentration of the analyte from complex matrices. Due to the polar character and the reduced possibility of detection by UV-VIS absorption of the analytes, the project will also focus on the derivatization of the analytes in order to increase hydrophobicity and fluorescent labelling. This procedure will be performed after the extraction of the analytes from the samples by means of MIPs. The project is oriented towards industrial applications and the consortium will take important steps to prepare for the successful commercialisation of the developed MIPs, such as: (1) the development and optimisation at laboratory level will take into account not only the analytical performance but also the requirements to adapt the synthesis to industrial production in large quantities, (2) the production of the developed MIPs will be tested at industrial reactor level by the SME that will continue the production after the project is completed and (3) the developed MIPs will be tested by a laboratory specialised in chemical analysis in order to assess the fulfilment of end-user requirements.