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National Institute of Research and Development for Biological Sciences

New safe phytoextracts from different species of Carpathian trees with improvement potential of the animal and/or human health

Description

The main goal of the project is to obtain phytoextracts from the bark of scorus (Sorbus aucuparia L.) and elm (Ulmus glabra Huds.), with a rich content in polyphenols, by means of a validated extraction technology, able to improve the health of animals and/or humans. The identification and characterisation of the pharmacological properties of the new phytoextracts will allow to outline the spectrum of their use as antioxidant, antimutagenic, antitumour or healing agents, with increased biocompatibility. The extraction technology, validated in a previous project, with superior extraction efficiency and yield of polyphenolic fractions from the bark of some woody species (Rosa canina, Hippophae rhamnoides, Vitis vinifera), will be used, adapted and validated for the production of phytocompounds with a rich polyphenol content from autochthonous Carpathian species of scorus and elm. The experimental model necessary to identify and quantify the specific pharmacological properties of the extracts from scorus and elm will be based on in vitro cellular models (normal and neoplastic) to: identify the antitumour effect (cytotoxicity, clonogenicity); determine the pro- or antioxidant activity; evaluate the impact on genomic integrity; evaluate the healing property; outline the mechanisms of action (apoptosis, cell cycle, proliferation); evaluate the biocompatibility of these phytoproducts. On the basis of the most important and eloquent pharmacological valences, the possible directions of valorisation in the human/animal health sphere will be suggested.