Bogdan Bucur
CS I - Centrul de Bioanaliză
Biografie
Am absolvit Universitatea din București, Facultatea de Chimie, specializarea Chimie, obținând diploma de chimist în anul 1998. În 2001, am obținut diploma de master la aceeași universitate, în cadrul Facultății de Chimie, specializarea Biosenzori în monitorizarea mediului. În perioada 2000-2005, am urmat studii doctorale la Facultatea de Chimie, Universitatea din București, și la BIOMEM, Centrul de Fitofarmacie, Universitatea din Perpignan, Franța, obținând titlul de doctor. Teza mea de doctorat a avut ca temă “Senzori chimici și biochimici cu aplicații în analiza în flux”.
Am dobândit experiență în domeniul cercetării în chimia analitică, concentrându-mă pe dezvoltarea de biosenzori enzimatici pentru analize de mediu și alimente, metode electrochimice de analiză și caracterizare a suprafețelor, modificarea electrozilor cu mediatori, membrane, compuși chimici și enzime. De asemenea, am lucrat cu metode electrogravimetrice de analiză, utilizând ADN și lectine drept componente biologice specifice pentru recunoașterea analitului. Am acumulat, de asemenea, experiență în cinetica enzimatică, inhibiția enzimatică și stabilizarea enzimelor. Am fost director/reponsabil pentru mai multe proiecte.
Publicatii
| Publication | Authors | Date | |
|---|---|---|---|
article
4-Phenyl Isothiocyanate Modified Electrode Based On Diazonium Electrodeposition For Heavy Metals Detection In Pharmaceuticals |
Radulescu MC; Bucur MP; Bucur B; Iosageanu A; Radu GL | Talanta, 2025 | |
RezumatA stable layer of 4-phenyl isothiocyanate (4-PITC) was successfully electrografted on glassy carbon electrode (GCE). The electrode modification process was performed in three sequential steps in a single solution: (1) reduction by chronoamperometry of the nitro groups of 4-NITC to amino moieties (2) in situ reaction of amino with nitrous acid to obtain unstable diazonium functional groups and (3) electrografting by chronopotentiometry of diazonium. A thin optimal layer with minimum surface fouling was obtained. The formation of the organic film was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The electrode was used for detection of Pb(II) and Fe(II) by differential pulse voltammetry (DPV). Under optimal conditions, the limit of detection 1.2 mu g L-1 for Pb(II) and of 2.0 mu g L-1 for Fe(II) were achieved. The developed electrode was successfully applied to detect Pb(II) and Fe(II) in injectable iron pharmaceuticals used to treat iron deficiency anemia simultaneous with investigation of contamination by Pb(II). |
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article
Acetylcholinesterase And Butyrylcholinesterase Co-Immobilized On A Copper Containing Prussian Blue Modified Electrode For The Broad Screening Of Insecticides |
Bucur M.-P.; Radulescu M.-C.; Bucur B.; Radu G.L. | Analytical And Bioanalytical Chemistry, 2024 | |
RezumatWe have developed a bienzymatic biosensor that contains acetylcholinesterase together with butyrylcholinesterase co-immobilized on the same electrode modified with a stabilized copper containing Prussian blue electrodeposited on electrodes coated with 4-aminothiophenol monolayer using diazonium chemistry and copper nanoparticles for improved sensitivity. There are organophosphorus and carbamate neurotoxic insecticides that inhibit only one of the two enzymes, e.g., pirimicarb inhibits butyrylcholinesterase at much lower concentrations than acetylcholinesterase while methomyl inhibits only acetylcholinesterase. Our system is simple and in a single measurement provides a sensitive signal for insecticides’ presence based on the inhibition of the enzyme with the highest affinity for each toxic compound. The limits of detection are 50 ng/mL pirimicarb for the bienzymatic biosensor in comparison with 400 ng/mL pirimicarb for the acetylcholinesterase biosensor and 6 ng/mL methomyl for the bienzymatic biosensor, while inhibition is obtained for the butyrylcholinesterase biosensor at 700 ng/mL. Graphical Abstract: (Figure presented.) © The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature 2024. |
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article
Cavitation-Effect-Based Treatments And Extractions For Superior Fruit And Milk Valorisation |
Bucur Madalina-Petruta; Radulescu Maria-Cristina; Radu Gabriel Lucian; Bucur Bogdan | Molecules, 2023 | |
RezumatUltrasound generates cavities in liquids with high-energy behaviour due to large pressure variations, leading to (bio)chemical effects and material modification. Numerous cavity-based treatments in food processes have been reported, but the transition from research to industrial applications is hampered by specific engineering factors, such as the combination of several ultrasound sources, more powerful wave generators or tank geometry. The challenges and development of cavity-based treatments developed for the food industry are reviewed with examples limited to two representative raw materials (fruit and milk) with significantly different properties. Both active compound extraction and food processing techniques based on ultrasound are taken into consideration. |
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article
Electrochemical Detection Of Heavy Metals In Raw Materials Using A Glassy Carbon Electrode Modified With 4-Aminothiophenol |
Radulescu Maria-Cristina; Bucur Madalina-Petruta; Bucur Bogdan; Radu Gabriel Lucian | Journal Of The Electrochemical Society, 2022 | |
RezumatSensitive and stable electrodes modified with 4-aminothiophenol were developed for heavy metal detection based on coordination bonds between thiol moieties and analyte. The electrodes were modified using diazonium chemistry in one step modification protocol. The presence of 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) in the electrodeposition media avoids the formation of multilayers and improves the performances due to low surface passivation and good electrochemical transfer at the interface with the solution. Electrodeposition of the organic layer on the electrode surface by chronopotentiometry allowed a good control of the electrode modification process, avoids passivation, and leads to reproducible layers with improved characteristics in comparison with modifications carried out by cyclic voltammetry or chronoamperometry. The electrode was able to detect Pb(II) by differential pulse voltammetry with a linear range of 2.5-400 mu g l(-1) and the detection limit of 1.2 mu g l(-1) and Cd(II) with the calibration line in the range of 2.5-400 mu g l(-1) and a detection limit of 1.5 mu g l(-1). Low interferences were observed and the electrodes were applied for analysis of real samples: fruits pomace and waters. (C) 2022 The Electrochemical Society (ECS). Published on behalf of ECS by IOP Publishing Limited. |
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article
Ternary Water-Organic Solvent Mixtures Used For Insecticide Spe Extraction And Analysis With Acetylcholinesterase Biosensor |
Bucur Madalina-Petruta; Bucur Bogdan; Bacalum Elena; David Victor; Radu Gabriel Lucian | Analytical Biochemistry, 2022 | |
RezumatFew biosensors are reported for usage in combination with the organic solvent due to their negative impact on the enzymes. The usage of ternary water-organic solvent mixtures in combination with acetylcholinesterase biosensors allows to increase the useable total content of organic solvents with minimum negative effects to a higher content in comparison with a single organic solvent in water. The combination of acetonitrile/ethanol/ water has a smaller negative effect on both enzyme activity and inhibition by insecticides in comparison with acetonitrile/methanol/water mixtures. The insecticides were eluted from solid-phase extraction (SPE) columns with a binary mixture of organic solvents acetonitrile/ethanol in 1/3 ratio and subsequently analysed with an acetylcholinesterase biosensor and the optimum total content of organic solvents of 12%. The analytical method allows the analysis of complex samples with improved selectivity and at improved limits of detection for chlorpyrifos-oxon and carbofuran analysis in river waters and soil samples. The usage of mixtures of organic solvents in combination with enzymes is an interesting approach that allows working with a higher total content of organic solvents than each individual solvent. |
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article
Ternary Water–Organic Solvent Mixtures Used For Insecticide Spe Extraction And Analysis With Acetylcholinesterase Biosensor |
Bucur M.-P.; Bucur B.; Bacalum E.; David V.; Radu G.L. | Analytical Biochemistry, 2022 | |
RezumatFew biosensors are reported for usage in combination with the organic solvent due to their negative impact on the enzymes. The usage of ternary water–organic solvent mixtures in combination with acetylcholinesterase biosensors allows to increase the useable total content of organic solvents with minimum negative effects to a higher content in comparison with a single organic solvent in water. The combination of acetonitrile/ethanol/water has a smaller negative effect on both enzyme activity and inhibition by insecticides in comparison with acetonitrile/methanol/water mixtures. The insecticides were eluted from solid-phase extraction (SPE) columns with a binary mixture of organic solvents acetonitrile/ethanol in 1/3 ratio and subsequently analysed with an acetylcholinesterase biosensor and the optimum total content of organic solvents of 12%. The analytical method allows the analysis of complex samples with improved selectivity and at improved limits of detection for chlorpyrifos-oxon and carbofuran analysis in river waters and soil samples. The usage of mixtures of organic solvents in combination with enzymes is an interesting approach that allows working with a higher total content of organic solvents than each individual solvent. © 2022 Elsevier Inc. |
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article
Addressing The Selectivity Of Enzyme Biosensors: Solutions And Perspectives |
Bucur Bogdan; Purcarea Cristina; Andreescu Silvana; Vasilescu Alina | Sensors, 2021 | |
RezumatEnzymatic biosensors enjoy commercial success and are the subject of continued research efforts to widen their range of practical application. For these biosensors to reach their full potential, their selectivity challenges need to be addressed by comprehensive, solid approaches. This review discusses the status of enzymatic biosensors in achieving accurate and selective measurements via direct biocatalytic and inhibition-based detection, with a focus on electrochemical enzyme biosensors. Examples of practical solutions for tackling the activity and selectivity problems and preventing interferences from co-existing electroactive compounds in the samples are provided such as the use of permselective membranes, sentinel sensors and coupled multi-enzyme systems. The effect of activators, inhibitors or enzymatic substrates are also addressed by coupled enzymatic reactions and multi-sensor arrays combined with data interpretation via chemometrics. In addition to these more traditional approaches, the review discusses some ingenious recent approaches, detailing also on possible solutions involving the use of nanomaterials to ensuring the biosensors' selectivity. Overall, the examples presented illustrate the various tools available when developing enzyme biosensors for new applications and stress the necessity to more comprehensively investigate their selectivity and validate the biosensors versus standard analytical methods. |
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article
Investigation Of The Corrosion Inhibition Properties Of New Phenyl Aldehyde Organic Layers Functionalized With Different Amino Alcohols Electrodeposited On Copper |
Chira Ana; Bucur Bogdan; Radu Gabriel-Lucian | Comptes Rendus Chimie, 2021 | |
RezumatPhenyl aldehyde layers were formed on copper foils by electro-assisted diazonium reactions and subsequently functionalized with either amino-2-propanol or 2-amino-1-butanol. The anticorrosion properties of the two obtained organic films 2-[(phenylmethylidene)amino]butan-1-ol and (phenylmethylidene)amino-propan-2-ol were evaluated in aerated buffer acetate solution pH = 3.5. The electrochemical corrosion inhibition properties were investigated after different immersion times using electrochemical impedance spectroscopy and potentiodynamic polarization analysis (Tafel). Contact angle measurement confirms the hydrophobic nature of the new organic coatings and layer stability after immersion in buffer. The results showed that both developed layers provided good anticorrosion protection and the highest inhibition was achieved using 2-[(phenylmethylidene)amino]butan-1-ol coating on copper, which makes it a useful device for anticorrosion protection. Moreover, correlations between the molecular structures of the corrosion inhibitors and their protecting efficiencies have been established. |
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article
Investigation Of The Corrosion Inhibition Properties Of New Phenyl Aldehyde Organic Layers Functionalized With Different Amino Alcohols Electrodeposited On Copper [Etude Des Propriétés D'Inhibition De La Corrosion Par De Nouvelles Couches Organiques De Phénylaldéhyde Fonctionnaliseés Avec Différents Amino-Alcools Électrodéposés Sur Cuivre] |
Chira A.; Bucur B.; Radu G.-L. | Comptes Rendus Chimie, 2021 | |
RezumatPhenyl aldehyde layers were formed on copper foils by electro-assisted diazonium reactions and subsequently functionalized with either amino-2-propanol or 2-amino-1-butanol. The anticorrosion properties of the two obtained organic films 2-[(phenylmethylidene)amino]butan-1-ol and (phenylmethylidene)amino-propan-2-ol were evaluated in aerated buffer acetate solution pH Æ 3.5. The electrochemical corrosion inhibition properties were investigated after different immersion times using electrochemical impedance spectroscopy and potentiodynamic polarization analysis (Tafel). Contact angle measurement confirms the hydrophobic nature of the new organic coatings and layer stability after immersion in buffer. The results showed that both developed layers provided good anticorrosion protection and the highest inhibition was achieved using 2-[(phenylmethylidene)amino]butan-1-ol coating on copper, which makes it a useful device for anticorrosion protection.Moreover, correlations between the molecular structures of the corrosion inhibitors and their protecting efficiencies have been established. © 2021 Elsevier Masson SAS. All rights reserved. |
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article
Investigation Of The Corrosion Inhibition Properties Of New Phenyl Aldehyde Organic Layers Functionalized With Different Amino Alcohols Electrodeposited On Copper; [Etude Des Propriétés D'Inhibition De La Corrosion Par De Nouvelles Couches Organiques De Phénylaldéhyde Fonctionnaliseés Avec Différents Amino-Alcools Électrodéposés Sur Cuivre] |
Chira A.; Bucur B.; Radu G.-L. | Comptes Rendus Chimie, 2021 | |
RezumatPhenyl aldehyde layers were formed on copper foils by electro-assisted diazonium reactions and subsequently functionalized with either amino-2-propanol or 2-amino-1-butanol. The anticorrosion properties of the two obtained organic films 2-[(phenylmethylidene)amino]butan-1-ol and (phenylmethylidene)amino-propan-2-ol were evaluated in aerated buffer acetate solution pH Æ 3.5. The electrochemical corrosion inhibition properties were investigated after different immersion times using electrochemical impedance spectroscopy and potentiodynamic polarization analysis (Tafel). Contact angle measurement confirms the hydrophobic nature of the new organic coatings and layer stability after immersion in buffer. The results showed that both developed layers provided good anticorrosion protection and the highest inhibition was achieved using 2-[(phenylmethylidene)amino]butan-1-ol coating on copper, which makes it a useful device for anticorrosion protection.Moreover, correlations between the molecular structures of the corrosion inhibitors and their protecting efficiencies have been established. © 2021 Elsevier Masson SAS. All rights reserved. |
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