Corneliu Vrancianu
CS - Danubius
Biografie
Publicatii
| Publication | Authors | Date | |
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article
From Microbial Ecology To Clinical Challenges: The Respiratory Microbiome'S Role In Antibiotic Resistance |
Niculescu Adelina-Gabriela; Mitache Mihaela Magdalena; Grumezescu Alexandru Mihai; Chifiriuc Mariana Carmen; Mihai Mara Madalina; Tantu Monica Marilena; Tantu Ana Catalina; Popa Loredana Gabriela; Grigore Georgiana Alexandra; Cristian Roxana-Elena; Popa Mircea Ioan; Vrancianu Corneliu Ovidiu | Pathogens, 2025 | |
RezumatAntibiotic resistance represents a growing public health threat, with airborne drug-resistant strains being especially alarming due to their ease of transmission and association with severe respiratory infections. The respiratory microbiome plays a pivotal role in maintaining respiratory health, influencing the dynamics of antibiotic resistance among airborne pathogenic microorganisms. In this context, this review proposes the exploration of the complex interplay between the respiratory microbiota and antimicrobial resistance, highlighting the implications of microbiome diversity in health and disease. Moreover, strategies to mitigate antibiotic resistance, including stewardship programs, alternatives to traditional antibiotics, probiotics, microbiota restoration techniques, and nanotechnology-based therapeutic interventions, are critically presented, setting an updated framework of current management options. Therefore, through a better understanding of respiratory microbiome roles in antibiotic resistance, alongside emerging therapeutic strategies, this paper aims to shed light on how the global health challenges posed by multi-drug-resistant pathogens can be addressed. |
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article
From Microbial Ecology To Clinical Challenges: The Respiratory Microbiome’S Role In Antibiotic Resistance |
Niculescu A.-G.; Mitache M.M.; Grumezescu A.M.; Chifiriuc M.C.; Mihai M.M.; Tantu M.M.; Tantu A.C.; Popa L.G.; Grigore G.A.; Cristian R.-E.; Popa M.I.; Vrancianu C.O. | Pathogens, 2025 | |
RezumatAntibiotic resistance represents a growing public health threat, with airborne drug-resistant strains being especially alarming due to their ease of transmission and association with severe respiratory infections. The respiratory microbiome plays a pivotal role in maintaining respiratory health, influencing the dynamics of antibiotic resistance among airborne pathogenic microorganisms. In this context, this review proposes the exploration of the complex interplay between the respiratory microbiota and antimicrobial resistance, highlighting the implications of microbiome diversity in health and disease. Moreover, strategies to mitigate antibiotic resistance, including stewardship programs, alternatives to traditional antibiotics, probiotics, microbiota restoration techniques, and nanotechnology-based therapeutic interventions, are critically presented, setting an updated framework of current management options. Therefore, through a better understanding of respiratory microbiome roles in antibiotic resistance, alongside emerging therapeutic strategies, this paper aims to shed light on how the global health challenges posed by multi-drug-resistant pathogens can be addressed. © 2025 by the authors. |
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article
From Rock To Living Systems: Lanthanides Toxicity And Biological Interactions |
Constantin Marian; Chioncel Mariana F.; Petrescu Livia; Vrancianu Corneliu Ovidiu; Paun Mihaela; Cristian Roxana-Elena; Sidoroff Manuela; Dionisie Mihai Viorel; Chifiriuc Mariana Carmen | Ecotoxicology And Environmental Safety, 2025 | |
RezumatSince the discovery of lanthanides, the expanding range of applications and the growing demand for lanthanides in different aspects of life have escalated their dispersion in the environment, raising concerns about their impact on the living world. This review explores the interaction between lanthanides and different groups of living organisms (bacteria, algae, lichens, plants, invertebrates, and low vertebrates), reflecting the current state of scientific knowledge. We have aimed to provide a comprehensive overview of relevant studies, highlight existing gaps, and suggest potential areas for future research to enhance the understanding of this topic. |
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article
Healthcare-Associated Infections: The Role Of Microbial And Environmental Factors In Infection Control-A Narrative Review |
Sandu Andreea M.; Chifiriuc Mariana C.; Vrancianu Corneliu O.; Cristian Roxana-E.; Alistar Cristina F.; Constantin Marian; Paun Mihaela; Alistar Alexandru; Popa Loredana G.; Popa Mircea I.; Tantu Ana C.; Sidoroff Manuela E.; Mihai Mara M.; Marcu Andreea; Popescu George; Tantu Monica M. | Infectious Diseases And Therapy, 2025 | |
RezumatHealthcare-associated infections (HAIs), previously known as nosocomial infections, represent a significant threat to healthcare systems worldwide, prolonging patient hospital stays and the duration of antimicrobial therapy. One of the most serious consequences of HAIs is the increase in the rate of antibiotic resistance (AR) generated by the prolonged, frequent, and sometimes incorrect use of antibiotics, which leads to the selection of resistant bacteria, making treatment difficult and expensive, with direct consequences for the safety of patients and healthcare personnel. Therefore, timely and accurate diagnosis of HAIs is mandatory to develop appropriate infection prevention and control practices (IPC) and new therapeutic strategies. This review aimed to present the prevalence, risk factors, current diagnosis, including artificial intelligence (AI) and machine learning approaches, future perspectives in combating HAIs causative bacteria (phage therapy, microbiome-based interventions, and vaccination), and HAIs surveillance strategies. Also, we discussed the latest findings regarding the relationships of AR with climate change and environmental pollution in the context of the One Health approach. Phage therapy is an emerging option that can offer an alternative to ineffective antibiotic treatments for antibiotic-resistant bacteria causing HAIs. Clinical trials dealing with vaccine development for resistant bacteria have yielded conflicting results. Two promising strategies, fecal microbiota transplantation and probiotic therapy, proved highly effective against recurrent Clostridium difficile infections and have been shown to reduce HAI incidence in hospitalized patients undergoing antibiotic therapy. Artificial intelligence and machine learning systems offer promising predictive capabilities in processing large volumes of clinical, microbiological, and patient data but require robust data integration. Our paper argues that HAIs are still a global challenge, requiring stringent IPC policies, computer vision, and AI-powered tools. Despite promising avenues like integrated One Health approaches, optimized phage therapy, microbiome-based interventions, and targeted vaccine development, several knowledge gaps in clinical efficacy, standardization, and pathogen complexity remain to be answered. |
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article
Healthcare-Associated Infections: The Role Of Microbial And Environmental Factors In Infection Control—A Narrative Review |
Sandu A.M.; Chifiriuc M.C.; Vrancianu C.O.; Cristian R.-E.; Alistar C.F.; Constantin M.; Paun M.; Alistar A.; Popa L.G.; Popa M.I.; Tantu A.C.; Sidoroff M.E.; Mihai M.M.; Marcu A.; Popescu G.; Tantu M.M. | Infectious Diseases And Therapy, 2025 | |
RezumatHealthcare-associated infections (HAIs), previously known as nosocomial infections, represent a significant threat to healthcare systems worldwide, prolonging patient hospital stays and the duration of antimicrobial therapy. One of the most serious consequences of HAIs is the increase in the rate of antibiotic resistance (AR) generated by the prolonged, frequent, and sometimes incorrect use of antibiotics, which leads to the selection of resistant bacteria, making treatment difficult and expensive, with direct consequences for the safety of patients and healthcare personnel. Therefore, timely and accurate diagnosis of HAIs is mandatory to develop appropriate infection prevention and control practices (IPC) and new therapeutic strategies. This review aimed to present the prevalence, risk factors, current diagnosis, including artificial intelligence (AI) and machine learning approaches, future perspectives in combating HAIs causative bacteria (phage therapy, microbiome-based interventions, and vaccination), and HAIs surveillance strategies. Also, we discussed the latest findings regarding the relationships of AR with climate change and environmental pollution in the context of the One Health approach. Phage therapy is an emerging option that can offer an alternative to ineffective antibiotic treatments for antibiotic-resistant bacteria causing HAIs. Clinical trials dealing with vaccine development for resistant bacteria have yielded conflicting results. Two promising strategies, fecal microbiota transplantation and probiotic therapy, proved highly effective against recurrent Clostridium difficile infections and have been shown to reduce HAI incidence in hospitalized patients undergoing antibiotic therapy. Artificial intelligence and machine learning systems offer promising predictive capabilities in processing large volumes of clinical, microbiological, and patient data but require robust data integration. Our paper argues that HAIs are still a global challenge, requiring stringent IPC policies, computer vision, and AI-powered tools. Despite promising avenues like integrated One Health approaches, optimized phage therapy, microbiome-based interventions, and targeted vaccine development, several knowledge gaps in clinical efficacy, standardization, and pathogen complexity remain to be answered. © The Author(s) 2025. |
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article
Analysis Of The Nutrient Profile In Organic Manure From Romanian Animal Farms |
Jaga Ioana-Mihaela; Postolache Carmen; Botez Florina; Barbuceanu Florica; Romascu Luminita Maria; Marutescu Luminita; Popa Marcela; Vrancianu Corneliu Ovidiu | Journal Of Central European Agriculture, 2024 | |
RezumatThe livestock industry has a harmful effect on the environment and contributes to climate change by producing significant amounts of greenhouse gases such as carbon dioxide (CO2), nitrous oxide (N2O), methane (CH4), and ammonia (NH3). In line with this, the production and composting of manure is an important issue in livestock farming. During composting, the manure undergoes physical and nutritional profile changes, which can affect the environment. In this context, in the present study, we have investigated the dynamics of the chemical parameters such as pH, humidity (U%), organic matter (MO%), ammonium nitrogen N-NH4+(g/kg), nitrogen in the form of nitrite N-NO2-(g/kg), nitrate nitrogen N-NO3- (g/kg) and phosphorus phosphate P-PO4 3- (g/kg) of organic manure from cattle and swine farms during composting. The results revealed a pH variation between 6.9 and 8.23 during composting, which is normal for compost, as mentioned in the literature. A high water content of over 90% was preserved in organic manure and compost because the samples were very liquid. Nutrient values showed increased ammonium and total inorganic nitrogen in cattle farms and one pig farm during composting. This study highlights a rise in nutrient values, especially of ammonium and total inorganic nitrogen in both cattle and pig farms, an aspect that reinforces the need to evaluate intervention strategies and develop a set of recommendations leading to the reduction of exposure risks and a subsequent reduction in human health impacts. |
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article
Analysis Of The Nutrient Profile In Organic Manure From Romanian Animal Farms; [Analiza Profilului Nutrienților Din Gunoiul De Grajd Procesat Din Fermele De Animale Din România] |
Jagă I.-M.; Postolache C.; Botez F.; Bărbuceanu F.; Romașcu L.M.; Măruțescu L.; Popa M.; Vrâncianu C.O. | Journal Of Central European Agriculture, 2024 | |
RezumatThe livestock industry has a harmful effect on the environment and contributes to climate change by producing significant amounts of greenhouse gases such as carbon dioxide (CO2), nitrous oxide (N2 O), methane (CH4), and ammonia (NH3). In line with this, the production and composting of manure is an important issue in livestock farming. During composting, the manure undergoes physical and nutritional profile changes, which can affect the environment. In this context, in the present study, we have investigated the dynamics of the chemical parameters such as pH, humidity +-(U%), organic matter (MO%), ammonium nitrogen N-NH4 (g/kg), nitrogen in the form of nitrite N-NO2 (g/kg), nitrate-nitrogen N–NO3 (g/kg) and phosphorus phosphate P-PO3-4 (g/kg) of organic manure from cattle and swine farms during composting. The results revealed a pH variation between 6.9 and 8.23 during composting, which is normal for compost, as mentioned in the literature. A high water content of over 90% was preserved in organic manure and compost because the samples were very liquid. Nutrient values showed increased ammonium and total inorganic nitrogen in cattle farms and one pig farm during composting. This study highlights a rise in nutrient values, especially of ammonium and total inorganic nitrogen in both cattle and pig farms, an aspect that reinforces the need to evaluate intervention strategies and develop a set of recommendations leading to the reduction of exposure risks and a subsequent reduction in human health impacts. © 2024, University of Zagreb - Faculty of Agriculture. All rights reserved. |
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article
Decoding The Resistome, Virulome And Mobilome Of Clinical Versus Aquatic Acinetobacter Baumannii In Southern Romania |
Gheorghe-Barbu Irina; Surleac Marius; Barbu Ilda Czobor; Paraschiv Simona; Banica Leontina Mirela; Rotaru Liviu-Iulian; Vrancianu Corneliu Ovidiu; Lazar Mihai Nita; Otelea Dan; Chifiriuc Mariana Carmen | Heliyon, 2024 | |
RezumatAcinetobacter baumannii, a notorious opportunistic pathogen, presents a formidable challenge in both clinical and environmental fields due to its resilience and ability to acquire resistance. This study undertook a comprehensive analysis of 183 A. baumannii isolates collected between 2019 and 2022 from intra-hospital infections (IHI), hospital sewages (Hs), wastewater treatment plants (WWTP), and adjacent river waters from two Southern cities, focusing on their resistome, virulome, and mobilome through isolation on chromogenic media, identification by MALDI-TOF-MS and antibiotic susceptibility testing by disk diffusion) followed by genotypic characterization [Whole Genome Sequencing (WGS), 3rd generation sequencing through the MinION (ONT) platform, pangenome description, and respectively horizontal gene transfer through conjugation assays]. Our findings reveal significant genomic plasticity and the prevalence of high-risk international clones, underlining the potential of these isolates to act as reservoirs for antibiotic resistance genes (ARGs) that could be dynamically exchanged between clinical and environmental settings through mobile genetic elements (MGEs) such as the pMAL1 plasmids and the critical role of WWTPs in the persistence and spread of A. baumannii. Moreover, our study presents the first report of the co-occurrence of blaOXA-23 and blaOXA-72 in A. baumannii ST2 clone. Thus, our research underscores the necessity for integrated surveillance and targeted interventions across healthcare and environmental sectors to mitigate the risk posed by this adaptable pathogen. |
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article
Enterobacterales Carrying Chromosomal Ampc Β-Lactamases In Europe (Euescpm): Epidemiology And Antimicrobial Resistance Burden From A Cohort Of 27 Hospitals, 2020-2022 |
Boattini Matteo; Bianco Gabriele; Llorente Laura Iglesias; Acero Laura Alonso; Nunes Daniel; Seruca Miguel; Mendes Vasco Santos; Almeida Andre; Bastos Paulo; Rodriguez-Villodres Angel; Gascon Adelina Gimeno; Halperin Ana Veronica; Canton Rafael; Escartin Maria Nieves Larrosa; Gonzalez-Lopez Juan Jose; Floch Pauline; Massip Clemence; Chainier Delphine; Barraud Olivier; Dortet Laurent; Cuzon Gaelle; Zancanaro Clement; Mizrahi Assaf; Schade Rogier; Rasmussen Asger Nellemann; Schonning Kristian; Hamprecht Axel; Schaffarczyk Lukas; Gloeckner Stefan; Roedel Juergen; Kristof Katalin; Balonyi Agnes; Mancini Stefano; Quiblier Chantal; Fasciana Teresa; Giammanco Anna; Paglietti Bianca; Rubino Salvatore; Budimir Ana; Bedenic Branka; Rubic Zana; Marinovic Jelena; Gartzonika Konstantina; Christaki Eirini; Mavromanolaki Viktoria Eirini; Maraki Sofia; Yalcin Tugba Yanik; Azap Oezlem Kurt; Licker Monica; Musuroi Corina; Talapan Daniela; Vrancianu Corneliu Ovidiu; Comini Sara; Zalas-Wiecek Patrycja; Michalska Anna; Cavallo Rossana; Cristino Jose Melo; Costa Cristina | International Journal Of Antimicrobial Agents, 2024 | |
RezumatIntroduction: The ESCPM group ( Enterobacter species including Klebsiella aerogenes - formerly Enterobacter aerogenes, Serratia species, Citrobacter freundii complex, Providencia species and Morganella morganii ) has not yet been incorporated into systematic surveillance programs. Methods: We conducted a multicentre retrospective observational study analysing all ESCPM strains isolated from blood cultures in 27 European hospitals over a 3 -year period (2020-2022). Diagnostic approach, epidemiology, and antimicrobial susceptibility were investigated. Results: Our study comprised 6,774 ESCPM isolates. MALDI-TOF coupled to mass spectrometry was the predominant technique for bacterial identification. Susceptibility to new beta -lactam/ beta -lactamase inhibitor combinations and confirmation of AmpC overproduction were routinely tested in 33.3% and 29.6% of the centres, respectively. The most prevalent species were E. cloacae complex (4 4.8%) and S. marcescens (22.7%). Overall, third -generation cephalosporins (3GC), combined third- and fourthgeneration cephalosporins (3GC + 4GC) and carbapenems resistance phenotypes were observed in 15.7%, 4.6%, and 9.5% of the isolates, respectively. AmpC overproduction was the most prevalent resistance mechanism detected (15.8%). Among carbapenemase-producers, carbapenemase type was provided in 44.4% of the isolates, VIM- (22.9%) and OXA-48-enzyme (16%) being the most frequently detected. E. cloacae complex, K. aerogenes and Providencia species exhibited the most notable cumulative antimicrobial resistance profiles, with the former displaying 3GC, combined 3GC + 4GC and carbapenems resistance phenotypes in 15.2%, 7.4%, and 12.8% of the isolates, respectively. K. aerogenes showed the highest rate of both 3GC resistant phenotype (29.8%) and AmpC overproduction (32.1%), while Providencia species those of both carbapenems resistance phenotype (42.7%) and carbapenemase production (29.4%). ESCPM isolates exhibiting both 3GC and combined 3GC + 4GC resistance phenotypes displayed high susceptibility to ceftazidime/avibactam (98.2% and 95.7%, respectively) and colistin (90.3% and 90.7%, respectively). Colistin emerged as the most active drug against ESCPM species (except those intrinsically resistant) displaying both carbapenems resistance phenotype (85.8%) and carbapenemase production (97.8%). Conclusions: This study presented a current analysis of ESCPM species epidemiology in Europe, providing insights to inform current antibiotic treatments and guide strategies for antimicrobial stewardship and diagnostics. (c) 2024 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ ) |
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article
Insights Into The Effects Of Lanthanides On Mammalian Systems And Potential Applications |
Constantin Marian; Chifiriuc Mariana Carmen; Vrancianu Corneliu Ovidiu; Petrescu Livia; Cristian Roxana-Elena; Crunteanu Ioana; Grigore Georgiana Alexandra; Chioncel Mariana F. | Environmental Research, 2024 | |
RezumatLanthanides, a group of elements with unique chemical properties, have garnered significant attention for their varied biological effects, ranging from cytotoxic to protective, depending on concentration, cell type, and exposure conditions. This review provides a detailed examination of the biological interactions of lanthanides with mammalian systems, including humans, by exploring their impact on different cell lines and organisms. Through a systematic assessment of current research, this work highlights the dual nature of lanthanides, identifying them as both potential therapeutic agents and environmental toxins. Furthermore, it underscores the importance of understanding their mechanisms to mitigate health risks, particularly for those exposed occupationally or via environmental sources. The review concludes with an overview of knowledge gaps and future research directions necessary for unlocking the therapeutic potential of lanthanides while ensuring safety and sustainability in their applications. |
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