Craita Roșu
CS II - Biologie Experimentală și Aplicată
Publicatii
| Publication | Authors | Date | |
|---|---|---|---|
article
In vitro cytophysiological response induced by three food additives on some mammalian cell line models |
Gherghel D., Mihai C.-T., Vochita G., Rosu C. M. | Journal of Experimental and Molecular Biology, 2024 | |
RezumatThis research is focused on cellular response to sodium metabisulphite (E223), sodium benzoate (E211), and sodium nitrite (E250) application on two normal mammalian cells, namely MCF-12A (ATCC CRL-3598) and Vero (ATCC CCL-81). The monitored parameters were cell viability (MTT assay), cell morphology (optical microscopy) and cell survival (clonogenic assay). The treatment was applied in doses of 12.5; 25; 50 and 100 µg/mL. According to all tests assessed, our results proved a dose-response relationship, the most sensibility presented the MCF-12A cell line. |
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article
Nodules Of Medicago Spp. Host A Diverse Community Of Rhizobial Species In Natural Ecosystems |
Stefan Andrei; Van Cauwenberghe Jannick; Rosu Craita Maria; Stedel Catalina; Chan Crystal; Simms Ellen L.; Iticescu Catalina; Tsikou Daniela; Flemetakis Emmanouil; Efrose Rodica Catalina | Agronomy-Basel, 2024 | |
RezumatBiological nitrogen fixation by rhizobia-nodulated legumes reduces the dependence on synthetic nitrogen fertilizers. Identification of locally adapted rhizobia may uncover economically valuable strains for sustainable agriculture. This study investigated the diversity and symbiotic potential of rhizobia associated with three Medicago species from Eastern Romania's ecosystems. Phenotypic screening ensured that only rhizobial species were retained for molecular characterization. 16S rDNA sequencing clustered the isolates into four distinct groups: Sinorhizobium meliloti, Sinorhizobium medicae, Rhizobium leguminosarum, and Mesorhizobium spp. The chromosomal genes (atpD, glnII, recA) and nifH phylogenies were congruent, while the nodA phylogeny grouped the Mesorhizobium spp. isolates with R. leguminosarum. Medicago sativa was the most sampled plant species, and only S. meliloti and R. leguminosarum were found in its nodules, while Medicago falcata nodules hosted S. meliloti and Mesorhizobium spp. Medicago lupulina was the only species that hosted all four identified rhizobial groups, including S. medicae. This study provides the first report on the Mesorhizobium spp. associated with M. falcata nodules. Additionally, R. leguminosarum and two Mesorhizobium genospecies were identified as novel symbionts for Medicago spp. Comparative analysis of Medicago-associated rhizobia from other studies revealed that differences in 16S rDNA sequence type composition were influenced by Medicago species identity rather than geographic region. |
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article
Effects Of Non-Thermal Plasma Activated Water And Low Temperature On Wheat Sprouts: A Focus On Photosynthetic Pigments, Protein And Phenolic Contents, Antioxidant Activity, Antioxidant And Prooxidant Enzymes Activity |
Mandici Alexandru; Rosu Craita; Burlica Radu; Cretu Daniel Eusebiu; Astanei Dragos; Beniuga Oana; Topa Denis Constantin; Aostacioaei Tudor George; Miron Anca | Farmacia, 2022 | |
RezumatThe study aimed to evaluate the effects of non-thermal plasma activated water (PAW) in combination with low temperature (15 degrees C) on the biosynthetic potential, antioxidant activity and enzymes activity in wheat (Triticum aestivum L. cv. Glosa) sprouts. The exposure to three different types of PAW (PAW1, PAW2 or PAW3) and low temperature (15 degrees C) had positive influence on wheat sprouts. Content in proteins and photosynthetic pigments and activity of superoxide dismutase increased in wheat sprouts at day 3 and shoots at day 8. Peroxidase was stimulated in wheat sprouts (day 3) while catalase activation was detected in shoots (day 8). Polyphenol oxidase, responsible for enzymatic browning, was inhibited in shoots at day 8. PAW1 boosted free phenolic content in shoots at day 8. An increment in the antioxidant activity of bound phenolic fraction of shoots (day 8) under treatment with PAW1 was also detected. Overall, non-thermal PAW in combination with low temperature (15 degrees C) is an efficient approach to enhance the quality of wheat (Triticum aestivum L. cv. Glosa) sprouts. |
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article
Increasing Rdi Outputs Through The Competitive Research Funding Operational Programme With Impact On The Emerging Market |
Dobrota Carmen; Rosu Maria-Magdalena; Puiu Andreea-Ionela; Milea Eduard C.; Paun Mihaela | Romanian Statistical Review, 2022 | |
RezumatConsidering the broad impact of applied research on the economy, RDI funding evaluations are required both in terms of the number of allocated resources and the management of these resources. RDI efficacy depends on human resources productivity and the financing instruments established through national priorities. This paper offers an analysis of the RDI investments in Romania allocated through the Competitiveness Operational Programme 2014-2020, covering the European Structural and Investment Funds, namely the European regional development fund. The analysis of the funded projects highlighted the common trends among the beneficiaries of RDI projects, their options in managing resources in relation to the eligibility of costs, and their national distribution between the seven development regions of Romania. The amount of funding was discussed in terms of the smart specialization domains established by the national strategy. The conclusions of the study, correlated with the objectives set by the SNCDI 2014-2020, are relevant for the management of the future funding instruments allocated to RDI by the ERDF in the period 2021-2027. |
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article
New Methodological Approach To Classify Educational Institutions-A Case Study On Romanian High Schools |
Necula Marian; Rosu Maria-Magdalena; Firescu Alexandra-Maria; Basu Cecilia; Ardelean Andreea; Milea Eduard C.; Paun Mihaela | Mathematics, 2022 | |
RezumatSince 2021, the National Evaluation exam in Romania (the exam aimed to assess 14- to 15-year-old students' knowledge at the end of lower secondary education and just before high school) has presented a novel examination structure that resembles PISA tests. The current investigation analyses the 2021 National Evaluation exam results compared to the results obtained in the previous two years (2019-2020) as an evaluation of upper education institutions' effectiveness in Romania. The results put forward the same conclusions as proposed by extant literature on Bucharest high schools. Even though the educational institutions show apparent progress and great adaptability to change, a more in-depth analysis reveals great inequality between educational institutions. As in the case of Bucharest, nationally there are only a small number of top-performing high schools in Romania, with the majority of high schools ranking in the lowest category as conceptualised in the study. The current investigation puts together a novel methodology for classification based on the main instruments proposed in literature: a letter grade classification and Turner's f-index. The results and the methodological proposal are especially relevant considering the latest PISA (2018) conclusions on Romania characterising the national educational system as underperforming. |
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article
Preliminary Study On The Impact Of Non-Thermal Plasma Activated Water On The Quality Of Triticum Aestivum L. Cv. Glosa Sprouts |
Mandici Alexandru; Cretu Daniel Eusebiu; Burlica Radu; Astanei Dragos; Beniuga Oana; Rosu Craita; Topa Denis Constantin; Aostacioaei Tudor George; Aprotosoaie Ana Clara; Miron Anca | Horticulturae, 2022 | |
RezumatThe present study aimed to investigate the effects of non-thermal plasma-activated water (PAW) on Triticum aestivum L. cv. Glosa sprouts. Two types of PAW were generated by exposing distilled water to a high-voltage electric discharge. Wheat caryopses were treated either with PAW1 (25 mg/L NO3-, 4 mg/L NO2-, and 6 mg/L H2O2) or PAW2 (35 mg/L NO3-, 5 mg/L NO2-, and 7.5 mg/L H2O2) for 8 days, with samples being collected at days 1, 2, 3, and 8. The germination rate, growth parameters, protein, photosynthetic pigments, total phenolic contents, antioxidant activity of free and bound phenolic fractions, and activity of antioxidant enzymes were evaluated. Both PAW1 and PAW2 had positive effects on Triticum aestivum L. cv. Glosa sprouts. PAW2 had a better impact on sprouts with respect to growth parameters, free phenolic content, and antioxidant activity of the free phenolic fraction (day 3), the accumulation of proteins, and photosynthetic pigments (day 8). In conclusion, exposure to PAW increases the quality of Triticum aestivum L. cv. Glosa sprouts. Further optimization of PAW treatment is required for the most favorable impact on the accumulation of biomass and health-promoting compounds in sprouts. |
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article
Secondary Metabolites From Artemisia Genus As Biopesticides And Innovative Nano-Based Application Strategies |
Ivanescu Bianca; Burlec Ana Flavia; Crivoi Florina; Rosu Craita; Corciova Andreia | Molecules, 2021 | |
RezumatThe Artemisia genus includes a large number of species with worldwide distribution and diverse chemical composition. The secondary metabolites of Artemisia species have numerous applications in the health, cosmetics, and food sectors. Moreover, many compounds of this genus are known for their antimicrobial, insecticidal, parasiticidal, and phytotoxic properties, which recommend them as possible biological control agents against plant pests. This paper aims to evaluate the latest available information related to the pesticidal properties of Artemisia compounds and extracts and their potential use in crop protection. Another aspect discussed in this review is the use of nanotechnology as a valuable trend for obtaining pesticides. Nanoparticles, nanoemulsions, and nanocapsules represent a more efficient method of biopesticide delivery with increased stability and potency, reduced toxicity, and extended duration of action. Given the negative impact of synthetic pesticides on human health and on the environment, Artemisia-derived biopesticides and their nanoformulations emerge as promising ecofriendly alternatives to pest management. |
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article
Total polyphenols, flavonoids contents and antioxidant activity of Rosa sp. genotipes from different altitude of Romanian regions |
Oprica L., Rosu C.M. | Journal of Experimental and Molecular Biology, 2021 | |
RezumatEight wild rose hip genotypes from different altitudes varying from 3m to 902m were analyzed in order to evaluate the total polyphenols, flavonoids content and the antioxidant activity. The Rosa spp.rosehips collected from the Northeastern and the Southeasternof Romanianwere, as follows: Rosa canina, R. caesia, R. corymbifera, R. micrantha, R. nitidula, R. rubiginosa, R. subcanina, and R. vosagiaca. In some genotypes, the level of flavonoids and polyphenol content increased with the increasing altitude while in other it was observed a decrease. Polyphenol content reached a maximum of 144.36 mg GAE/g DW in R. rubiginosa whereas the lowest content of 61.72 mg GAE/g DW was recorded in R. caesia. The highest polyphenol content was reached at altitude of 860 m. The amount of flavonoids content ranged between 7.32 mg CE/g DW and 19.45 CE/g DW in R. caesia and in R. nitidula, respectively. The radical scavenging capacities of Rosa genotypes extracts were not positively correlated with altitude, except the R. corymbifera extractswhere the antioxidant activity increased with the increase of altitude. |
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article
Essential Oils Of Moldavian Thymus Species: Chemical Composition, Antioxidant, Anti-Aspergillus And Antigenotoxic Activities |
Aprotosoaie Ana Clara; Miron Anca; Ciocarlan Nina; Brebu Mihai; Rosu Craita Maria; Trifan Adriana; Vochita Gabriela; Gherghel Daniela; Luca Simon Vlad; Nita Alexandru; Costache Irina-Iuliana; Mihai Cosmin Teodor | Flavour And Fragrance Journal, 2019 | |
RezumatThymus species are important aromatic, medicinal and culinary plants with a significant economic value. This study evaluated for the first time the chemical composition and in vitro bioactivities of the essential oils (EOs) from five Moldavian Thymus species (T. vulgaris, T. x citriodorus, T. calcareus) and cultivars (T. vulgaris 'Faustini', T. citriodorus 'Aureus'). The main compounds in Thymus EOs were: thymol in T. vulgaris and T. calcareus EOs (55.44% and 55.45%, respectively), lavandulol in T. x citriodorus EO (54.27%), and geraniol in T. citriodorus 'Aureus' and T. vulgaris 'Faustini' EOs (60.31% and 31.45%, respectively). T. vulgaris and T. calcareus EOs showed the most potent antioxidant activities (EC50=0.003 mg/mL in ABTS radical cation scavenging assay) and exhibited significant inhibitory effects against aflatoxin-producing Aspergillus flavus fungus (MIC=0.25 mu L/mL). At doses that provided micromolar concentrations of thymol, T. vulgaris and T. calcareus EOs acted genoprotective at preventive and interventional levels against H2O2-induced genomic damage in V79 cells, the former being more active (6.21% and 5.52% vs. 25.13% and 7.26% tail DNA in pre- and post-treatment protocols, respectively). The genoprotective effects may be ascribed to antioxidant potential and, possibly, to stimulation of DNA repair processes. The Moldavian Thymus species are valuable resources of bioactive EOs for pharmaceutical and food industries (T. vulgaris, T.calcareus) but also for flavor industry and perfumery (T. x citriodorus, T. citriodorus 'Aureus', T. vulgaris 'Faustini'). |
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article
Performances Of Pichia Kudriavzevii In Decolorization, Biodegradation, And Detoxification Of Ci Basic Blue 41 Under Optimized Cultural Conditions |
Rosu Craita Maria; Vochita Gabriela; Mihasan Marius; Avadanei Mihaela; Mihai Cosmin Teodor; Gherghel Daniela | Environmental Science And Pollution Research, 2019 | |
RezumatThe aim of this study was to evaluate the performances of Pichia kudriavzevii CR-Y103 yeast strain for the decolorization, biodegradation, and detoxification of cationic dye C.I. Basic Blue 41, a toxic compound to aquatic life with long-lasting effects. Under optimized cultural conditions (10.0-gL(-1) glucose, 0.2-gL(-1) yeast extract, and 1.0-gL(-1) (NH4)(2)SO4), the yeast strain was able to decolorize 97.86% of BB41 (50mgL(-1)) at pH 6 within 4h of incubation at 30 degrees C under shaken conditions (12,238.00-gh(-1) average decolorization rate) and 100% within 12h. The UV-Vis spectral analysis, high-performance liquid chromatography (HPLC), and Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the complete decolorization and degradation of the BB41 dye by P. kudriavzevii CR-Y103. Also, other seven yeast strains, isolated from soil, as P. kudriavzevii (CR-Y108, CR-Y119, and CR-Y112), Candida tropicalis CR-Y128, Cyberlindnera saturnus CR-Y125, and Candida solani CR-Y124 have shown a promising decolorizing potential of azo-dye BB41 (99.89-76.09% decolorization). Phytotoxicity, cytotoxicity, and genotoxicity assays on Trifolium pratense and Triticum aestivum seedlings confirmed the high toxicity of BB41 dye (500ppm), with inhibition on germination rate (%), root and shoot elongation, decreasing of mitoxic index value (with 34.03% in T. pratense and 40.25% in T. aestivum), and increasing the frequency of chromosomal aberrations (6.87 times in T. pratense and 6.25 times in T. aestivum), compared to control. The same biomarkers indicated the nontoxic nature of the BB41 degraded metabolite (500ppm) obtained after P. kudriavzevii CR-Y103 treatment. Moreover, the healthy monkey kidney cells (Vero cells) had a low sensitivity to BB41 biodegraded products (250gmL(-1)) (MTT cell viability assay) and revealed minor DNA damage (comet assay) compared to BB41 dye treatment. These findings show that P. kudriavzevii could be used in eco-friendly bioremediation technologies, applicable for reducing the toxicity of basic azo-dyes containing wastewaters. |
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