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This study aimed to evaluate the hypoglycemic potential, antioxidant activity and prebiotic activity of a hydroalcoholic extract of blueberry pomace (BP), an aqueous extract of chia seeds (CS) and a novel combination of BP-CS extracts (BCM) for further use as ingredient of functional food. Spectrometric and HPLC analyses were used to characterize the total phenolic and flavonoid content and composition of BP, while CS was analyzed for total carbohydrate content. Data showed that the BCM mixture exerted an inhibition of alpha-amylase activity, which was 1.36 times higher than that of BP and 1.25 higher than CS extract. The mixture also showed better scavenging activity of free DPPH radicals than individual extracts, and had an IC50 value of 603.12 mu g/mL. In vitro testing indicated that both serum- and colon-reaching products of simulated intestinal digestion of BCM presented the capacity to protect Caco-2 intestinal cells against oxidative stress by inhibition of reactive oxygen species production. In addition, the colon-reaching product of BCM digestion had the capacity to significantly (p < 0.05) stimulate the growth of Lactobacillus rhamnosus and Lactobacillus acidophilus, revealing a prebiotic potential. All these results indicated that improved biological activity of the novel combination of BP and CS extracts could be due to the synergistic action of constituents. The combination is recommended for further testing and the development of novel functional food for controlling type 2 diabetes and gastrointestinal conditions.

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The aim of this work was to obtain a mixture of polyphenols and polysaccharides, extracted from forest fruit pomace (a side stream of forest fruit juice production) and chia seeds with a synergic antioxidant activity that will be further encapsulated and used in a fortified protein bar. We also intended to demonstrate the in vitro antioxidant effect, including in cell cultures, of the synergistic mixture encapsulated and subjected to a simulated digestion process.

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The paper presents some aspects regarding the obtaining of new ecological fertilizers for organic and classical agriculture by exploiting valuable residual bioresources, such as marine biomass and waste from viticulture and secondary vinification process. Biomass composed of marine green and brown algae, invertebrate shells, fermented and unfermented grape marc and chopped woody shoots of vines were combined in different proportions, resulting in eight experimental variants of fertilizer, which were incorporated into steppe chernozemic mollisols, specific to the Murfatlar area of Constan.a County, Romania. In 2019, in the proposed mixtures of soil - residual biomass, under greenhouse conditions, different types of vegetal species were cultivated, namely, Vitis vinifera L. (Pinot Gris variety), Lolium perene L., Sinapis alba L., and Trifolium repens L. The comparative statistical interpretation of the obtained results indicated a significant increase of essential nutrients ( phosphorus and potassium), classifying the fertilized soils into the category of those with very good nutrients supply. An improvement of the soil organic matter (humus) was observed, the increase compared to the control being of 40.9% in the case of the V5 variant. A moderate increase of the 3% CaCO3 content was also observed. Considering the experimental results obtained under greenhouse conditions, we will continue the study on experimental lots cultivated with different white and black varieties of Vitis vinifera L, representative for the Murfatlar vineyard.

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